Plasmid

Part:BBa_K3666102:Design

Designed by: Victor So   Group: iGEM20_Pittsburgh   (2020-10-27)


TlpA Temperature sensitive dimer regulating mWasabi expression


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

For our design, we required a promoter that would respond to the change in temperature triggerd by our magnetic nanoparticles. At specific high temperatures, which we work to maintain with alternating magnetic fields, we are able to harness TlpA's function as a promoter to initiate gene expression in E. coli. This is connected with a mWasabi protein to act as a reporter.


Source

TlpA comes from S. Typhimurium, mWasabi comes from Clavularia

References